参与保护离体大鼠脑微血管免受过氧化损伤的酶系统的发育变化。

Developmental changes in enzymatic systems involved in protection against peroxidation in isolated rat brain microvessels.

作者信息

Buard A, Clement M, Bourre J M

机构信息

INSERM U.26, Hôpital Fernand Widal, Paris, France.

出版信息

Neurosci Lett. 1992 Jul 6;141(1):72-4. doi: 10.1016/0304-3940(92)90337-7.

DOI:10.1016/0304-3940(92)90337-7

PMID:1354847

Abstract

Activities of 3 enzymes involved in the major detoxification pathway for peroxides were assessed in rat brain microvessels. Between the 7th and 60th day after birth, glutathione peroxidase specific activity remained constant in microvessels, while glutathione reductase specific activity increased from day 14 to day 60. On the other hand, the specific activity of these two enzymes evolved similarly in total brain homogenate: they increased between day 7 and day 30, and then reached a plateau. In contrast, catalase specific activity in microvessels was markedly decreased from day 7 to day 60. A significant decrease in this enzyme specific activity was also observed in brain homogenate during development. However, in microvessels, catalase specific activity remained higher than that of brain homogenate throughout the time period studied. Our results support the idea that enzymatic mechanisms against peroxidative damage are required in early age, and could be potent at the level of the blood-brain barrier.

摘要

在大鼠脑微血管中评估了参与过氧化物主要解毒途径的3种酶的活性。出生后第7天至第60天期间，谷胱甘肽过氧化物酶的比活性在微血管中保持恒定，而谷胱甘肽还原酶的比活性从第14天至第60天增加。另一方面，这两种酶的比活性在全脑匀浆中的变化相似：它们在第7天至第30天之间增加，然后达到平稳状态。相比之下，微血管中过氧化氢酶的比活性从第7天至第60天显著降低。在发育过程中，脑匀浆中该酶的比活性也出现了显著下降。然而，在整个研究期间，微血管中过氧化氢酶的比活性始终高于脑匀浆中的比活性。我们的结果支持这样一种观点，即针对过氧化损伤的酶促机制在幼年时期是必需的，并且在血脑屏障水平可能具有强大作用。