Ghatak S, Ho S M
Department of Biology, Tufts University, Medford, Massachusetts 02155, USA.
Biochem Biophys Res Commun. 1996 May 15;222(2):362-7. doi: 10.1006/bbrc.1996.0749.
In this investigation, the activities of five enzymes (catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, glucose-6-dehydrogenase) involved in oxygen radical (OR)-detoxification and antioxidative defense were measured in conjunction with an index of lipid peroxidation in major prostatic lobes of young (2 month-old) and old (9 month-old) rats. Selective decreases in catalase and glutathione peroxidase activities were observed in the ventral prostate lobe (VP), while a general decline in the activities of all five enzymes studied was noted in the dorsolateral prostate lobes (DLPs) of senescent rats. The extent of lipid peroxidation, measured as concentrations of thiobarbituric acid-reactive products, was greatly elevated in both VP and DLP of old rats. These results demonstrate for the first time that reduction in antioxidant capacity and elevation in peroxidative damage occur in rat prostate during aging. aging. We postulate that these prooxidative alterations promote diseases in the aging prostate.
在本研究中，我们测定了年轻（2月龄）和老年（9月龄）大鼠主要前列腺叶中参与氧自由基（OR）解毒和抗氧化防御的五种酶（过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、葡萄糖-6-脱氢酶）的活性，并结合脂质过氧化指标进行分析。在腹侧前列腺叶（VP）中观察到过氧化氢酶和谷胱甘肽过氧化物酶活性选择性降低，而在老年大鼠的背外侧前列腺叶（DLP）中，所研究的所有五种酶的活性均普遍下降。以硫代巴比妥酸反应产物浓度衡量的脂质过氧化程度在老年大鼠的VP和DLP中均显著升高。这些结果首次证明，衰老过程中大鼠前列腺的抗氧化能力降低，过氧化损伤增加。我们推测，这些促氧化改变会促进衰老前列腺中的疾病发生。
