NORMAN P S
J Exp Med. 1958 Jul 1;108(1):53-68. doi: 10.1084/jem.108.1.53.
The inactivation of purified plasmin by serum or plasma was studied using a caseinolytic assay for determination of plasmin. On the basis of kinetic evidence, the presence of two inhibitors in normal human or guinea pig serum or plasma is suggested. The first reacts immediately at any temperature, but is easily dissociable and does not combine with plasmin in a fixed ratio. The second combines more slowly with plasmin at a rate which depends on temperature and on the concentration of both plasmin and inhibitor. There is a fixed ratio of reaction between plasmin and the second inhibitor and the combination does not readily dissociate. There is sufficient of the "slow" inhibitor in normal plasma to inactivate more than 30 times its own content of potential plasmin. The presence of suitable substrate for plasmin tends to reverse the combination with "immediate" inhibitor and to stop the further action of "slow" inhibitor.
使用酪蛋白溶解测定法测定纤溶酶，研究了血清或血浆对纯化纤溶酶的灭活作用。基于动力学证据，提示正常人或豚鼠血清或血浆中存在两种抑制剂。第一种在任何温度下都能立即反应，但很容易解离，且不以固定比例与纤溶酶结合。第二种与纤溶酶结合较慢，其速率取决于温度以及纤溶酶和抑制剂的浓度。纤溶酶与第二种抑制剂之间存在固定的反应比例，且这种结合不易解离。正常血浆中存在足够的“慢”抑制剂，可使超过其自身潜在纤溶酶含量30倍的纤溶酶失活。纤溶酶合适底物的存在往往会逆转与“即时”抑制剂的结合，并阻止 “慢” 抑制剂的进一步作用。
