Akhtar M H, Mahadevan S
Centre for Food and Animal Research, Agriculture Canada, Ottawa, Ontario.
Drug Metab Dispos. 1992 May-Jun;20(3):356-9.
Incubation of 3-phenoxybenzoic acid[carbonyl-14C] with chicken kidney microsomal fraction produced two major radioactive compounds identified as 3-hydroxybenzoic acid and 4'-hydroxy-3-phenoxybenzoic acid. It was found that 4'-hydroxy-3-phenoxybenzoic acid was transitory in nature and was rapidly converted into 3-hydroxybenzoic acid. The conversion of 3-phenoxybenzoic acid to the products was NADPH dependent. This is a first example of metabolism of 4'-hydroxy-3-phenoxybenzoic acid to 3-hydroxybenzoic acid. Cytosol did not promote any cleavage. Similar activities were present in liver microsomes, but the activity was lower than in kidney. Intestinal contents, homogenates, or microsomal fractions did not metabolize 3-phenoxybenzoic acid.
用鸡肾微粒体组分对[羰基 - ¹⁴C]3 - 苯氧基苯甲酸进行孵育，产生了两种主要的放射性化合物，分别鉴定为3 - 羟基苯甲酸和4'-羟基 - 3 - 苯氧基苯甲酸。发现4'-羟基 - 3 - 苯氧基苯甲酸本质上是短暂存在的，并迅速转化为3 - 羟基苯甲酸。3 - 苯氧基苯甲酸向产物的转化依赖于NADPH。这是4'-羟基 - 3 - 苯氧基苯甲酸代谢为3 - 羟基苯甲酸的首个实例。胞质溶胶不促进任何裂解。肝微粒体中也存在类似活性，但活性低于肾中的活性。肠内容物、匀浆或微粒体组分均不能代谢3 - 苯氧基苯甲酸。
