McLure A R, Gordon J
Department of Microbiology, Gartnavel General Hospital, Glasgow, UK.
J Hosp Infect. 1992 Aug;21(4):291-9. doi: 10.1016/0195-6701(92)90139-d.
The in-vitro activity of povidone-iodine (PVP-I) and chlorhexidine (CHX) against 33 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) was evaluated by a quantitative suspension test method. Bactericidal potency was measured by the logarithmic reduction factors (LRFs) achieved with each strain, tested at dilutions 25-800 over exposure times 30-300 s using a challenge of approximately 10(7) colony forming units (cfu) ml-1. The mean LRFs achieved over all dilutions, times and strains were significantly higher for PVP-I than CHX. PVP-I exhibited a superior killing effect whether measured by rate of kill or final LRF achieved. This difference was highly significant as judged by analysis of variance (P less than 0.001). Full efficacy of an antiseptic has been defined as a safe LRF greater than five. Over the dilution range 25-200 this was achieved by CHX with only three of 33 strains. In contrast, PVP-I achieved full efficacy with all 33 strains.
采用定量悬液试验法评估了聚维酮碘（PVP-I）和氯己定（CHX）对33株耐甲氧西林金黄色葡萄球菌（MRSA）临床分离株的体外活性。通过各菌株在25至800倍稀释度、30至300秒暴露时间下，使用约10⁷菌落形成单位（cfu）/ml的菌悬液进行挑战试验所获得的对数减少因子（LRF）来测定杀菌效力。在所有稀释度、时间和菌株上，PVP-I获得的平均LRF显著高于CHX。无论通过杀灭速率还是最终获得的LRF来衡量，PVP-I均表现出更强的杀灭效果。经方差分析判断，这种差异具有高度显著性（P<0.001）。一种防腐剂的完全效力被定义为安全LRF大于5。在25至200的稀释范围内，CHX对33株菌株中的仅3株达到了这一标准。相比之下，PVP-I对所有33株菌株均达到了完全效力。
