显微分光光度测定法的双波长法。II. 一组便于计算的表格。
The two-wavelength method of microspectrophotometry. II. A set of tables to facilitate the calculations.
作者信息
MENDELSOHN M L
出版信息
J Biophys Biochem Cytol. 1958 Jul 25;4(4):415-24. doi: 10.1083/jcb.4.4.415.
The calculations required for two-wavelength measurements are time consuming and laborious. In order to circumvent this limitation of the method, a set of tables which combined four operations into one has been designed and is reproduced within. The tables are based on Patau's formulae. The two transmission readings obtained according to the photometric method provide the coordinates which lead directly to a value for the relative absorbance. The product of this absorbance and the area of the photometric field gives the relative amount of chromophore in the field. The range of transmission values covered in the table corresponds to the effective range of the two-wavelength method.
双波长测量所需的计算既耗时又费力。为了克服该方法的这一局限性，已设计了一组将四种运算合并为一种的表格，并在文中重现。这些表格基于帕陶公式。根据光度法获得的两个透射读数提供了直接得出相对吸光度值的坐标。该吸光度与光度场面积的乘积给出了该场中发色团的相对量。表格中涵盖的透射值范围与双波长法的有效范围相对应。
Zotero 插件