Franzén L E, Ghassemifar M R
Department of Pathology II, University Hospital, Linköping, Sweden.
Eur J Surg. 1992 Jun-Jul;158(6-7):333-7.
To quantify measures of healing in zinc-deficient and healthy rats.
Randomized study.
30 male Sprague-Dawley rats.
Zinc deficiency was induced in half the rats. All rats underwent laparotomy and standard perforations were made in the small intestinal mesentery with a scalpel. At 1, 3, 5, 7 and 10 days after operation 6 rats were killed by overdose of anaesthetic agents and the specimens of the mesentery were fixed.
Measurement of cellular volume density, surface density of the rough endoplasmic reticulum, and surface density of the plasma membrane.
Perforations started to close on day 4, and most were closed by day 10. Cellular volume density reached its peak between days 3 and 5, as did surface density of rough endoplasmic reticulum. There were no significant differences between the two groups for either measurement. The surface density of the rough endoplasmic reticulum, however, was significantly higher in controls than in zinc deficient animals on days 3-10 (p less than 0.001). The surface density of the plasma membrane was significantly higher in zinc-deficient animals on days 1-3 (p less than 0.04), and in control animals on days 5-10 (p less than 0.01).
Protein synthesis and formation of scar tissue were slightly lower in the zinc-deficient animals, and the higher plasma membrane surface density implies that contraction may be an important part of healing in the small intestinal mesentery in rats.
量化缺锌和健康大鼠的愈合指标。
随机研究。
30只雄性斯普拉格-道利大鼠。
半数大鼠被诱导缺锌。所有大鼠均接受剖腹手术，并用手术刀在小肠系膜上制作标准穿孔。术后第1、3、5、7和10天，过量麻醉剂处死6只大鼠，取系膜标本固定。
测量细胞体积密度、粗面内质网表面密度和质膜表面密度。
穿孔在第4天开始闭合，大多数在第10天闭合。细胞体积密度在第3天至第5天达到峰值，粗面内质网表面密度也是如此。两组在这两项测量中均无显著差异。然而，在第3至10天，对照组粗面内质网的表面密度显著高于缺锌动物（p<0.001）。在第1至3天，缺锌动物的质膜表面密度显著更高（p<0.04），而在第5至10天，对照动物的质膜表面密度显著更高（p<0.01）。
缺锌动物的蛋白质合成和瘢痕组织形成略低，较高的质膜表面密度表明收缩可能是大鼠小肠系膜愈合的重要组成部分。
