Wingren U, Franzén L, Larson G M, Malcherek P, Schultz G S
Department of Surgery, University of Louisville, School of Medicine, Kentucky 40292.
J Surg Res. 1992 Jul;53(1):48-54. doi: 10.1016/0022-4804(92)90012-o.
Epidermal growth factor (EGF) has been reported to stimulate healing of wounds in skin, cornea, and gastric mucosa. In the present study, we further investigate the effect of endogenous and exogenous EGF in healing of connective tissue wounds using the rat perforated mesentery model. Healing of mesenteric perforations is accomplished by the connective tissue fibroblasts since there are no interfering variables such as interactions of epithelial cells, desiccation, or foreign materials such as sutures or subcutaneous implants. We performed laparotomy in 114 adult male Sprague-Dawley rats and made 20 standardized perforations in the mesentery of each rat with a scalpel. Rats were randomly assigned to five groups. Group I received no treatment after surgery; Group II received intraperitoneal injections of phosphate-buffered saline (PBS) after surgery and then twice daily for the following 3 days; Group III received 10 micrograms of EGF in the PBS injections according to the same regimen as Group II; Group IV had sham exploration of the submandibular salivary glands; and Group V animals had excision of the submandibular glands 3 days before laparotomy to deprive the main source of EGF in rat. On Days 4 through 10 after surgery rats were sacrificed and the percentage of perforations in each rat which were closed was determined. The curves for the time course of wound closure for Groups IV and V were not different indicating that endogenous submandibular EGF does not play a role in healing of mesenteric wounds.(ABSTRACT TRUNCATED AT 250 WORDS)
据报道，表皮生长因子（EGF）可促进皮肤、角膜和胃黏膜伤口的愈合。在本研究中，我们使用大鼠穿孔肠系膜模型，进一步研究内源性和外源性EGF在结缔组织伤口愈合中的作用。肠系膜穿孔的愈合由结缔组织成纤维细胞完成，因为不存在上皮细胞相互作用、干燥或缝线或皮下植入物等异物等干扰变量。我们对114只成年雄性Sprague-Dawley大鼠进行剖腹手术，并用手术刀在每只大鼠的肠系膜上制作20个标准化穿孔。大鼠被随机分为五组。第一组术后不接受治疗；第二组术后接受腹腔注射磷酸盐缓冲盐水（PBS），然后在接下来的3天每天注射两次；第三组按照与第二组相同的方案在PBS注射中接受10微克EGF；第四组对下颌下唾液腺进行假探查；第五组动物在剖腹手术前3天切除下颌下腺，以剥夺大鼠EGF的主要来源。在术后第4天至第10天处死大鼠，测定每只大鼠中闭合穿孔的百分比。第四组和第五组伤口闭合时间进程曲线无差异，表明内源性下颌下EGF在肠系膜伤口愈合中不起作用。（摘要截短至250字）
