Franzén L, Ghassemifar R, Schultz G
Department of Pathology, Linköping University, Sweden.
Eur J Cell Biol. 1993 Apr;60(2):346-50.
Epidermal growth factor (EGF) is known to stimulate wound repair, including connective tissue repair as observed in the perforated rat mesentery. In the present study we assessed changes in expression of EGF receptors during healing of connective tissue by measuring the binding of [125I]EGF to perforated and unperforated mesenteric membranes. Autoradiographic grain density was measured on flat mounted or sectioned mesenteric tissue. Laparotomy alone caused an inflammatory reaction in the abdominal cavity and significantly (p < 0.04) increased the binding of [125I]EGF to unperforated membranes by 70% on days 1 and 3 postoperatively. In perforated mesenteric membranes, the binding of EGF in a 1 mm wide zone around the incision was significantly higher (p < 0.05) than EGF binding in the adjacent tissue on days 3 through 7. Furthermore, the average grain density in a 100 microns wide segment around the incision was approximately twice as high (p < 0.008) as the grain density in the mesentery 100 to 500 microns around the incision or in adjacent tissue (p < 0.004). These results indicate that expression of EGF receptor increases in the region of regenerating connective tissue and supports the hypothesis that EGF receptor plays a key role in mesenteric wound healing.
已知表皮生长因子（EGF）可刺激伤口修复，包括在大鼠穿孔肠系膜中观察到的结缔组织修复。在本研究中，我们通过测量[125I]EGF与穿孔和未穿孔肠系膜膜的结合，评估了结缔组织愈合过程中EGF受体表达的变化。在平铺或切片的肠系膜组织上测量放射自显影颗粒密度。仅开腹手术就会引起腹腔内的炎症反应，并在术后第1天和第3天使[125I]EGF与未穿孔膜的结合显著（p < 0.04）增加70%。在穿孔的肠系膜膜中，术后第3天至第7天，切口周围1毫米宽区域内的EGF结合显著高于相邻组织（p < 0.05）。此外，切口周围100微米宽段的平均颗粒密度大约是切口周围100至500微米肠系膜或相邻组织颗粒密度的两倍（p < 0.008）（p < 0.004）。这些结果表明，EGF受体在再生结缔组织区域的表达增加，并支持EGF受体在肠系膜伤口愈合中起关键作用的假说。
