Ceccatelli S, Seroogy K B, Millhorn D E, Terenius L
Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden.
Brain Res. 1992 Sep 4;589(2):225-30. doi: 10.1016/0006-8993(92)91281-i.
Immunofluorescence colocalization techniques were used to examine the extent of coexistence of the endogenous opioid peptide dynorphin with catecholamines and the related opioid peptide enkephalin within neurons of the rat medulla oblongata. Immunoreactivities for dynorphin and the catecholamine-synthesizing enzyme tyrosine hydroxylase were found to coexist within a limited subpopulation of A2 catecholamine cells, localized to the medial nucleus of the nucleus tractus solitarii. Colocalization of the two opioid peptides was found mainly within perikarya situated in the medial and ventrolateral nuclei of the nucleus tractus solitarii. Triple-labeling studies revealed only rare cases of catecholamine/dynorphin/enkephalin coexistence. These data demonstrate that dynorphin is present within a restricted subpopulation of catecholamine neurons in the dorsal medulla oblongata. In addition, the content of either of the opioids enkephalin or dynorphin appears to distinguish subsets of medullary catecholamine neurons.
采用免疫荧光共定位技术，研究内源性阿片肽强啡肽与儿茶酚胺以及相关阿片肽脑啡肽在大鼠延髓神经元内共存的程度。结果发现，强啡肽免疫反应性与儿茶酚胺合成酶酪氨酸羟化酶在位于孤束核内侧核的A2儿茶酚胺细胞的有限亚群中共存。两种阿片肽的共定位主要见于位于孤束核内侧核和腹外侧核的胞体。三重标记研究仅发现极少数儿茶酚胺/强啡肽/脑啡肽共存的情况。这些数据表明，强啡肽存在于延髓背侧儿茶酚胺神经元的一个受限亚群中。此外，脑啡肽或强啡肽中的任何一种含量似乎都能区分延髓儿茶酚胺神经元的亚群。
