Melander T, Hökfelt T, Rökaeus A, Cuello A C, Oertel W H, Verhofstad A, Goldstein M
J Neurosci. 1986 Dec;6(12):3640-54. doi: 10.1523/JNEUROSCI.06-12-03640.1986.
The coexistence of galanin (GAL)-like immunoreactivity (LI) with markers for catecholamines, 5-hydroxytryptamine (5-HT), GABA, or some neuropeptides was mapped in the rat CNS by using adjacent sections, as well as by elution-restaining and double-labeling immunocytochemistry. Many instances of coexistence were observed, but there were also numerous GAL-positive cell body populations displaying distributions similar to those of these markers but without apparent coexistence. In the hypothalamic arcuate nucleus GAL-LI was found in a large proportion of tyrosine hydroxylase (TH)-positive cell bodies (A12 cells), both in the dorsomedial and ventrolateral subdivisions, with a higher number in the latter. GAL-LI coexisted in glutamic acid decarboxylase (GAD)-positive somata in the posterior aspects of the arcuate nucleus and at all rostrocaudal levels in fibers in the external layer of the median eminence. In the anterior hypothalamus, a large population of the cells of the parvocellular and magnocellular paraventricular nuclei contained both GAL-LI and vasopressin-LI. Moreover, somata containing both GAD- and GAL-LI were seen lateral to the mammillary recess in the tuberal and caudal magnocellular nuclei. Some of the neurons of the caudal group were shown to project to the occipital cortex using combined retrograde tracing and immunofluorescence. With regard to mesencephalic and medullary catecholamine neurons, GAL-LI coexisted in a large proportion of the noradrenergic locus coeruleus somata (A6 cell group) and in the A4 group dorsolateral to the fourth ventricle, as well as in the caudal parts of the A2 group in the dorsal vagal complex. However, in more rostral parts of the latter, especially in the medial subdivision of the solitary tract nucleus, a very large population of GAL-IR small cell bodies was seen intermingling with catecholamine neurons, but they did not contain TH-LI. Furthermore, GAL-IR cell bodies coextensive with, but not coexisting in, TH-IR somata were seen in the C1 (epinephrine) horea in the ventrolateral medulla at the level of area postrema and in the most rostral aspects of the C1 group. Finally, 5-HT-positive cell bodies of the mesencephalic and medullary raphe nuclei and a subpopulation of coarse 5-HT nerve fibers in the hippocampus co-contained GAL-LI. The present results demonstrate that a GAL-like peptide is present in many systems containing other neuroactive compounds, including dopamine, norepinephrine, 5-HT, GABA, and vasopressin.(ABSTRACT TRUNCATED AT 400 WORDS)
通过使用相邻切片、洗脱再染色和双标记免疫细胞化学方法，在大鼠中枢神经系统中描绘了甘丙肽（GAL）样免疫反应性（LI）与儿茶酚胺、5-羟色胺（5-HT）、γ-氨基丁酸（GABA）或某些神经肽标记物的共存情况。观察到许多共存实例，但也有大量GAL阳性细胞体群体，其分布与这些标记物相似，但无明显共存现象。在下丘脑弓状核中，在背内侧和腹外侧亚区的大部分酪氨酸羟化酶（TH）阳性细胞体（A12细胞）中发现了GAL-LI，后者数量更多。GAL-LI共存于弓状核后部的谷氨酸脱羧酶（GAD）阳性胞体以及正中隆起外层纤维的所有 rostrocaudal 水平。在前下丘脑，小细胞和大细胞室旁核的大量细胞同时含有GAL-LI和血管加压素-LI。此外，在结节和尾侧大细胞核中乳头体隐窝外侧可见同时含有GAD-LI和GAL-LI的胞体。使用逆行追踪和免疫荧光联合方法显示，尾侧组的一些神经元投射到枕叶皮质。关于中脑和延髓儿茶酚胺神经元，GAL-LI共存于大部分去甲肾上腺素能蓝斑核胞体（A6细胞群）、第四脑室背外侧的A4群以及迷走神经复合体中A2群的尾部。然而，在后者更靠前的部分，特别是在孤束核内侧亚区，可见大量GAL免疫反应性小细胞体与儿茶酚胺神经元混合，但它们不含TH-LI。此外，在最后区水平的腹外侧延髓C1（肾上腺素）组以及C1组最靠前的部分，可见与TH免疫反应性胞体重合但不共存的GAL免疫反应性胞体。最后，中脑和延髓中缝核的5-HT阳性细胞体以及海马中一部分较粗的5-HT神经纤维同时含有GAL-LI。目前的结果表明，一种GAL样肽存在于许多包含其他神经活性化合物的系统中，包括多巴胺、去甲肾上腺素、5-HT、GABA和血管加压素。（摘要截短于400字）
