Kaur J, Jaswal V M, Nagpaul J P, Mahmood A
Department of Biochemistry, Panjab University, Chandigarh, India.
Nutrition. 1992 Sep-Oct;8(5):338-42.
Ethanol feeding to rats for 40 days enhanced (p < 0.001) the activities of alkaline phosphatase, sucrase, gamma-glutamyltransferase (GTP), and p-nitrophenyl (PNP)-beta-D-galactosidase (p < 0.05) with no change in leucine amino peptidase (LAP) and PNP-beta-D-glucosidase activities in intestine compared with control rats. The activities of alkaline phosphatase, sucrase, and GTP were diminished (p < 0.01) in ethanol-fed malnourished rats. There was no change in LAP activity, but the levels of glucosidase and galactosidase were elevated under these conditions. Brush-border sialic acid, fucose, hexose, and hexosamine contents were elevated in ethanol-fed protein-deficient animals. Ethanol administration to normally fed rats elevated the membrane sialic acid and hexose contents, reduced fucose content, and had no effect on brush-border hexosamine content compared with the control group. These results are in agreement with data on lectin binding to brush borders under these conditions. Alcohol ingestion reduced the incorporation of [14C]-glucosamine into brush borders in rats maintained on an 18% protein diet but augmented the incorporation of [14C]-glucosamine and [14C]-mannose in protein-malnourished membranes. These observations suggest that nutrition status influences the sensitivity of microvillus membrane glycosylation to ethanol feeding in rat intestine.
与对照大鼠相比，给大鼠喂食乙醇40天可增强（p < 0.001）碱性磷酸酶、蔗糖酶、γ-谷氨酰转移酶（GTP）和对硝基苯基（PNP）-β-D-半乳糖苷酶的活性（p < 0.05），而肠中亮氨酸氨肽酶（LAP）和PNP-β-D-葡萄糖苷酶的活性无变化。在喂食乙醇的营养不良大鼠中，碱性磷酸酶、蔗糖酶和GTP的活性降低（p < 0.01）。LAP活性没有变化，但在这些条件下，葡萄糖苷酶和半乳糖苷酶的水平升高。在喂食乙醇的蛋白质缺乏动物中，刷状缘唾液酸、岩藻糖、己糖和己糖胺含量升高。与对照组相比，给正常喂食的大鼠施用乙醇可提高膜唾液酸和己糖含量，降低岩藻糖含量，并且对刷状缘己糖胺含量没有影响。这些结果与在这些条件下凝集素与刷状缘结合的数据一致。摄入酒精可减少维持在18%蛋白质饮食的大鼠中[14C]-葡萄糖胺掺入刷状缘，但可增加蛋白质营养不良膜中[14C]-葡萄糖胺和[14C]-甘露糖的掺入。这些观察结果表明，营养状况会影响大鼠肠道微绒毛膜糖基化对乙醇喂养的敏感性。
