Kaur J, Nagpaul J P, Mahmood A
Department of Biochemistry, Panjab University, Chandigarh.
Indian J Med Res. 1994 Dec;100:289-94.
The effect of feeding ethanol daily for 40 days was studied on various brush border enzymes in rat intestine. Brush border alkaline phosphatase (AP), lactase, gamma-glutamyltranspeptidase (gamma-GTP), p-nitrophenyl (PNP)-beta-D-galactosidase (P < 0.01) and sucrase (P < 0.001) were significantly enhanced while leucine aminopeptidase and PNP-beta-D-glucosidase activities were unaltered in ethanol fed rats compared to the controls. Kinetic studies revealed that an increase in Vmax together with a decrease in affinity in case of gamma-GTP and an increase in Vmax for AP and sucrase were responsible for the observed stimulation of enzyme activities in ethanol administered rats. Significant changes in enzyme activities were observed in different populations of enterocytes along the crypt-villus unit in the ethanol fed animals. These observations suggest that ethanol feeding modifies the brush border enzymes in rat intestine but the underlying mechanisms seem to be distinct in differentiating enterocytes.
研究了连续40天每日喂食乙醇对大鼠肠道各种刷状缘酶的影响。与对照组相比，乙醇喂养的大鼠刷状缘碱性磷酸酶（AP）、乳糖酶、γ-谷氨酰转肽酶（γ-GTP）、对硝基苯基（PNP）-β-D-半乳糖苷酶（P<0.01）和蔗糖酶（P<0.）活性显著增强，而亮氨酸氨肽酶和PNP-β-D-葡萄糖苷酶活性未改变。动力学研究表明，γ-GTP的Vmax增加且亲和力降低，以及AP和蔗糖酶的Vmax增加，是乙醇给药大鼠中观察到的酶活性刺激的原因。在乙醇喂养动物的隐窝-绒毛单元中，不同群体的肠细胞中观察到酶活性有显著变化。这些观察结果表明，乙醇喂养会改变大鼠肠道中的刷状缘酶，但潜在机制在分化肠细胞中似乎有所不同。 001）
