Saha N, Tong M C, Tay J S, Jeyaseelan K, Humphries S E
Department of Paediatrics (Division of Genetics), National University of Singapore.
Clin Genet. 1992 Oct;42(4):164-70. doi: 10.1111/j.1399-0004.1992.tb03232.x.
Five restriction fragment length polymorphisms (RFLP) of the apo B gene and their association with serum lipids and apolipoprotein levels have been studied in 139 Chinese patients with angiographically confirmed CAD (mean age 56.2 +/- 0.8 years) and 154 healthy Chinese subjects (mean 44.0 +/- 1.0 years) of both sexes. The patient group had significantly higher levels of serum total and LDL cholesterol; and apo B (P < 0.001) and lower HDL cholesterol and apo A-I (P < 0.001 and < 0.01, respectively). The frequencies of the rarer alleles of the ins/del, XbaI and EcoRI (but not the PvuII and MspI) polymorphisms were significantly lower in the Chinese compared to those reported in Caucasians. There was no significant difference in allelic frequencies of the signal peptide region (Ins/Del), XbaI, MspI and EcoRI sites of the apo B gene between the patient and control groups. The frequency of the rarer allele of the PvuII RFLP was significantly lower in the CAD patients (P < 0.05) compared to that in the control group (0.05 vs 0.10). None of the DNA polymorphisms was associated with a significant influence on serum lipid and apolipoprotein levels in the patients with coronary artery disease.
对139例经血管造影证实患有冠心病的中国患者（平均年龄56.2±0.8岁）和154例中国健康受试者（平均44.0±1.0岁）进行了载脂蛋白B基因的五种限制性片段长度多态性（RFLP）及其与血脂和载脂蛋白水平相关性的研究。患者组的血清总胆固醇和低密度脂蛋白胆固醇水平显著较高；以及载脂蛋白B（P<0.001），而高密度脂蛋白胆固醇和载脂蛋白A-I水平较低（分别为P<0.001和<0.01）。与高加索人报道的相比，中国人中ins/del、XbaI和EcoRI（但不是PvuII和MspI）多态性的罕见等位基因频率显著较低。患者组和对照组之间载脂蛋白B基因信号肽区域（Ins/Del）、XbaI、MspI和EcoRI位点的等位基因频率没有显著差异。与对照组相比，CAD患者中PvuII RFLP的罕见等位基因频率显著较低（P<0.05）（0.05对0.10）。在冠心病患者中，没有一种DNA多态性与血脂和载脂蛋白水平有显著关联。
