Chen Yeda, Zeng Jingtang, Tan Yiqing, Feng Min, Qin Jiheng, Lin Meihua, Zhao Xiang, Zhao Xiaolei, Liang Yan, Zhang Naizun, Rao Shaoqi
Institute of Medical Systems Biology, and School of Public Health, Guangdong Medical University, No. 1 New City Avenue, Songshan Lake Science and Technology Industry Park, 523808, Dongguan, Guangdong, China.
Maoming People's Hospital, Maoming, Guangdong, China.
Wien Klin Wochenschr. 2016 Dec;128(23-24):890-897. doi: 10.1007/s00508-016-1072-z. Epub 2016 Sep 8.
The study was carried out to examine the association between apolipoprotein B (ApoB) EcoRI polymorphism (E vs. E) (rs1042031) and coronary heart disease (CHD) risk by systematically analyzing multiple independent studies.
The Hardy-Weinberg equilibrium (HWE) test was applied to assess genotype frequency distribution in healthy controls. The quality of the studies was assessed using the Newcastle-Ottawa scale (NOS). Power analysis was performed with Power and Precision V4 software. A fixed effect model was used because no deviation from homogeneity was found. Publication bias was quantified and examined with Begg's funnel plot test and Egger's linear regression method. The meta-analysis was performed by Stata 12.0 software.
A total of 21 eligible association studies were merged in this meta-analysis and the pooled sample consisted of 2994 CHD patients and 3258 healthy controls. No significant publication bias and heterogeneity were observed in these studies. The pooled odds ratio (OR) and 95% confidence interval (CI) of E vs. E were 1.18 (1.06-1.32). The pooled OR (95% CI) of E E + E E vs. E E was 1.18 (1.04-1.34).
This meta-analysis indicated that ApoB EcoRI confers a moderate risk for CHD and the E allele at this locus might be a susceptibility allele for the development of CHD.
通过系统分析多项独立研究，探讨载脂蛋白B（ApoB）EcoRI基因多态性（E vs. E）（rs1042031）与冠心病（CHD）风险之间的关联。
应用哈迪-温伯格平衡（HWE）检验评估健康对照中的基因型频率分布。使用纽卡斯尔-渥太华量表（NOS）评估研究质量。使用Power and Precision V4软件进行效能分析。由于未发现异质性偏差，因此采用固定效应模型。采用Begg漏斗图检验和Egger线性回归方法对发表偏倚进行量化和检验。通过Stata 12.0软件进行荟萃分析。
本荟萃分析共纳入21项符合条件的关联研究，合并样本包括2994例冠心病患者和3258例健康对照。这些研究中未观察到明显的发表偏倚和异质性。E vs. E的合并比值比（OR）及95%置信区间（CI）为1.18（1.06 - 1.32）。EE + EE vs. EE的合并OR（95%CI）为1.18（1.04 - 1.34）。
本荟萃分析表明，ApoB EcoRI基因多态性赋予冠心病中度风险，该位点的E等位基因可能是冠心病发生的易感等位基因。
