GroEL/GroES与DnaK/DnaJ热休克蛋白在防止大肠杆菌蛋白质错误折叠中的协同作用。
Cooperation of GroEL/GroES and DnaK/DnaJ heat shock proteins in preventing protein misfolding in Escherichia coli.
作者信息
Gragerov A, Nudler E, Komissarova N, Gaitanaris G A, Gottesman M E, Nikiforov V
机构信息
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow.
出版信息
Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10341-4. doi: 10.1073/pnas.89.21.10341.
Newly synthesized proteins aggregate extensively in Escherichia coli rpoH mutants, which are deficient in the heat shock proteins (hsp). Overproduction of either GroEL and GroES or DnaK and DnaJ prevents aggregation. If expressed together, the four hsp are effective at physiological concentrations. Our data suggest that the GroEL and GroES proteins and the DnaK and DnaJ proteins have complementary functions in the folding and assembly of most proteins.
新合成的蛋白质在大肠杆菌rpoH突变体中大量聚集，这些突变体缺乏热休克蛋白（hsp）。过量表达GroEL和GroES或DnaK和DnaJ可防止聚集。如果一起表达，这四种热休克蛋白在生理浓度下是有效的。我们的数据表明，GroEL和GroES蛋白以及DnaK和DnaJ蛋白在大多数蛋白质的折叠和组装中具有互补功能。
Zotero 插件