Epstein R J, Druker B J, Roberts T M, Stiles C D
Division of Cell and Molecular Biology, Dana-Farber Cancer Institute, Boston, MA.
Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10435-9. doi: 10.1073/pnas.89.21.10435.
We inoculated rabbits with synthetic phosphopeptides, duplicating a major autophosphorylation site of the c-erbB-2 protooncogene product. The rabbits produced antisera that, after reverse immunoaffinity purification, selectively recognize the erbB-2 protein in its enzymatically active configuration. These anti-phosphopeptide antisera identify a subset of erbB-2-positive human cell lines wherein the protein is constitutively active as a tyrosine kinase. Synthetic phosphopeptides incorporating informative protein phosphorylation sites may prove useful for generating antibodies that indicate the activation state of additional tyrosine kinases and perhaps other proteins phosphorylated on serine and threonine residues.
我们用合成磷酸肽对兔子进行接种，复制c-erbB-2原癌基因产物的一个主要自磷酸化位点。兔子产生了抗血清，经过反向免疫亲和纯化后，能选择性识别处于酶活性构型的erbB-2蛋白。这些抗磷酸肽抗血清识别出一部分erbB-2阳性人类细胞系，其中该蛋白作为酪氨酸激酶持续处于激活状态。包含信息丰富的蛋白质磷酸化位点的合成磷酸肽可能被证明有助于生成指示其他酪氨酸激酶以及可能其他在丝氨酸和苏氨酸残基上磷酸化的蛋白质激活状态的抗体。
