Morin P A, Moore J J, Woodruff D S
Department of Biology, University of California, San Diego, La Jolla 92093-0116.
Proc Biol Sci. 1992 Sep 22;249(1326):293-7. doi: 10.1098/rspb.1992.0117.
We describe a non-invasive method of determining the subspecies identity of common chimpanzees (Pan troglodytes), based on subspecies-specific sequence differences in the mitochondrial genome. This procedure involves the extraction of DNA from hair, the amplification of a short (410 base pair (b.p.)) segment of the non-coding displacement loop (D-loop) by the polymerase chain reaction (PCR), and subspecies identification based on rapid allele-specific oligonucleotide (ASO) probe dot-blot typing. This approach will contribute to: (i) the colony-level management of captive chimpanzees by enabling managers to recognize hybrids between subspecies and minimize outbreeding depression; (ii) the recognition of inappropriately matched individuals in comparative behavioural and experimental studies; and (iii) forensic questions surrounding the origin of illegally traded animals.
我们描述了一种基于线粒体基因组中特定亚种序列差异来确定普通黑猩猩（Pan troglodytes）亚种身份的非侵入性方法。该程序包括从毛发中提取DNA，通过聚合酶链反应（PCR）扩增非编码置换环（D-loop）的一个短片段（410碱基对（b.p.）），以及基于快速等位基因特异性寡核苷酸（ASO）探针斑点杂交分型进行亚种鉴定。这种方法将有助于：（i）通过使管理者能够识别亚种间的杂种并尽量减少远交衰退，对圈养黑猩猩进行群体水平的管理；（ii）在比较行为和实验研究中识别不匹配的个体；以及（iii）围绕非法交易动物来源的法医问题。
