Hoffman L A, Codner M A, Shuster B A, Bogossian N, Egozi L, Harper A D, Steinman R M, LaTrenta G S, Shires G T
Department of Surgery, New York Hospital-Cornell Medical Center, N.Y.
Plast Reconstr Surg. 1992 Dec;90(6):999-1006; discussion 1007-8.
In an effort to further define the immunologic mechanisms leading to acute composite-tissue allograft rejection, the migratory patterns of donor leukocytes were evaluated. Using a rat model, 52 orthotopic vascularized hindlimb transplants were performed in strains representing major histocompatibility mismatches. In order to evaluate the effect of allogeneic skin on limb rejection, all donor skin was removed in a second group of allografts. Recipient lymphoid organs were examined during the week following transplantation for antigen-presenting cells using a donor-specific class II monoclonal antibody. Donor leukocytes, with dendritic cell morphology, were identified in recipient spleen and lymph nodes draining the allograft. Significantly higher numbers of donor leukocytes were present during postoperative days 1 through 4 for both groups. Association of these important passenger leukocytes with host T-lymphocytes may represent the site of initiation of the immune response.
为了进一步明确导致急性复合组织同种异体移植排斥反应的免疫机制，对供体白细胞的迁移模式进行了评估。使用大鼠模型，在代表主要组织相容性不匹配的品系中进行了52例原位血管化后肢移植。为了评估同种异体皮肤对肢体排斥反应的影响，在第二组同种异体移植中切除了所有供体皮肤。在移植后的一周内，使用供体特异性II类单克隆抗体检查受体淋巴器官中的抗原呈递细胞。在受体脾脏和引流同种异体移植的淋巴结中鉴定出具有树突状细胞形态的供体白细胞。两组在术后第1天至第4天期间供体白细胞数量均显著更高。这些重要的过客白细胞与宿主T淋巴细胞的关联可能代表了免疫反应起始的部位。
