Codner M A, Shuster B A, Steinman R M, Harper A D, LaTrenta G S, Hoffman L A
Division of Plastic Surgery, New York Hospital-Cornell University Medical Center, NY 10021.
Ann Plast Surg. 1990 Nov;25(5):353-9. doi: 10.1097/00000637-199011000-00003.
This study was undertaken in an initial effort to characterize the immunology of extremity transplantation by examining the pattern and kinetics of leukocyte migration from rat limb transplants. Migration of donor leukocytes was evaluated by examining recipient lymphoid tissues with a donor-specific, anti-major histocompatibility complex, class I monoclonal antibody. Double-antibody, two-color labeling was used to localize donor cells to specific regions within these tissues. Donor leukocytes, with dendritic cell morphology, were found in the T-cell-rich areas of lymph nodes draining the allograft and spleen. The donor cells were present on postoperative days 1 through 3 but were not present on days 5 to 7. Donor leukocytes were not present in distant lymph nodes or liver. These findings indicate a migration of leukocytes, most likely the highly immunogenic dendritic cell, from rat limb transplants to the draining lymphoid tissues. Migration occurs shortly after transplantation and may lead to the sensitization of alloreactive T-cells.
本研究旨在通过检测大鼠肢体移植中白细胞迁移的模式和动力学，初步描绘肢体移植的免疫学特征。通过使用供体特异性抗主要组织相容性复合体I类单克隆抗体检测受体淋巴组织，评估供体白细胞的迁移情况。采用双抗体双色标记法将供体细胞定位到这些组织内的特定区域。在引流同种异体移植组织的淋巴结和脾脏中富含T细胞的区域发现了具有树突状细胞形态的供体白细胞。供体细胞在术后第1天至第3天存在，但在第5天至第7天不存在。供体白细胞在远处的淋巴结或肝脏中不存在。这些发现表明白细胞，很可能是高免疫原性的树突状细胞，从大鼠肢体移植部位迁移到引流淋巴组织。迁移在移植后不久发生，可能导致同种反应性T细胞致敏。
