Richters C D, van Gelderop E, du Pont J S, Hoekstra M J, Kreis R W, Kamperdijk E W
Department of Cell Biology and Immunology, Medical Faculty, Vrije Universiteit, Amsterdam, The Netherlands.
Transplantation. 1999 Mar 27;67(6):828-32. doi: 10.1097/00007890-199903270-00008.
After transplantation, donor dendritic cells (DC) migrating to the draining lymph node of the recipient are thought to play an important role in the initiation of graft rejection. In this study, we compared the in vivo migration of DC after allogeneic skin transplantation with that after congeneic skin transplantation.
A rat model was used with the PVG-RT7b rats as donor animals. These rats have leukocytes bearing an epitope of the leukocyte common antigen that can be recognized by the monoclonal antibody His 41. The cells of the allogeneic (ACI) and congeneic (PVG) recipient animals do not express this marker.
In both recipient rat strains, graft-derived His 41+ DC could be detected in the T cell areas of the draining lymph nodes after skin transplantation. However, the number of migrated His 41+ cells present was lower in the allogeneic recipients. Similar results were obtained when skin DC isolated from the PVG-RT7b rats were injected subcutaneously into the hind footpads of allogeneic and congeneic recipients. Although the numbers of migrated His 41+ DC present were lower, the lymph nodes of the allogeneic recipients were much more enlarged and the grafts were rejected which did not occur in the congeneic recipients.
The presence of donor-derived DC in the graft draining lymph nodes underlines the importance of the direct route of allo-activation. The lower numbers of migrated His 41+ DC in lymph nodes of allogeneic recipients may be the result of killing of the cells after presentation of the allo-antigens to the recipient T cells.
移植后，迁移至受体引流淋巴结的供体树突状细胞（DC）被认为在移植物排斥反应的起始过程中发挥重要作用。在本研究中，我们比较了同种异体皮肤移植后与同基因皮肤移植后DC在体内的迁移情况。
使用大鼠模型，以PVG-RT7b大鼠作为供体动物。这些大鼠的白细胞带有白细胞共同抗原的一个表位，可被单克隆抗体His 41识别。同种异体（ACI）和同基因（PVG）受体动物的细胞不表达该标志物。
在两种受体大鼠品系中，皮肤移植后均可在引流淋巴结的T细胞区域检测到移植物来源的His 41+ DC。然而，同种异体受体中迁移的His 41+细胞数量较少。当将从PVG-RT7b大鼠分离的皮肤DC皮下注射到同种异体和同基因受体的后足垫时，也获得了类似结果。尽管迁移的His 41+ DC数量较少，但同种异体受体的淋巴结明显肿大，移植物被排斥，而同基因受体中未发生这种情况。
移植引流淋巴结中存在供体来源的DC突出了同种异体激活直接途径的重要性。同种异体受体淋巴结中迁移的His 41+ DC数量较少可能是在将同种异体抗原呈递给受体T细胞后细胞被杀伤的结果。
