Boesken W H, Batsford S R, Schmitz-Hübner U, Schneider G
Res Exp Med (Berl). 1976 Feb 27;167(1):23-30. doi: 10.1007/BF02180285.
The quantity of urinary proteins and their molecular weight composition was analyzed in different experimental glomerulopathies using the SDS-PAA-electrophoresis. Masugi nephritis, heterologous and autologous immune complex nephritis as well as D-penicillamine induced glomerulonephritis were studied in rabbits, guinea pigs and rats. The procedure allows (1) to distinguish physiological from low grade glomerular proteinuria by their respective characteristic patterns in early disease stages (2) to follow up the disease course of individual animals without sacrifice and (3) to discriminate species specificity of physiological urinary proteins. It is recommended to use this technique of urinary protein analysis in experimental conditions, where mild glomerular damage is expected.
采用SDS-PAA电泳法对不同实验性肾小球疾病中尿蛋白的含量及其分子量组成进行了分析。在兔、豚鼠和大鼠中研究了Masugi肾炎、异源性和自身免疫性免疫复合物肾炎以及D-青霉胺诱导的肾小球肾炎。该方法能够：（1）在疾病早期通过各自的特征模式区分生理性蛋白尿和轻度肾小球蛋白尿；（2）在不处死动物的情况下跟踪个体动物的病程；（3）区分生理性尿蛋白的物种特异性。建议在预期有轻度肾小球损伤的实验条件下使用这种尿蛋白分析技术。
