Gean P W, Huang C C, Lin J H, Tsai J J
Department of Pharmacology, College of Medicine, National Cheng-Kung University, Tainan City, Taiwan.
Brain Res. 1992 Oct 30;594(2):331-4. doi: 10.1016/0006-8993(92)91146-6.
The effect of isoproterenol (Iso) on synaptic transmission mediated by the N-methyl-D-aspartate (NMDA) receptors (EPSPNMDA) was investigated in slices of rat amygdala using intracellular recording techniques. EPSPNMDA was isolated pharmacologically by application of a solution containing the non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 microM) and GABAA receptor blocker bicuculline (20 microM). Superfusion of Iso (15 microM) produced a long-lasting enhancement of EPSPNMDA. Pretreatment the slices with propranolol (10 microM) completely prevented the effect of Iso confirming the mediation by beta-adrenergic receptors. These results provide the direct evidence for adrenergic modulation of excitatory amino acid neurotransmission in the vertebrate central nervous system.
利用细胞内记录技术，在大鼠杏仁核切片中研究了异丙肾上腺素（Iso）对由N-甲基-D-天冬氨酸（NMDA）受体介导的突触传递（EPSPNMDA）的影响。通过应用含有非NMDA受体拮抗剂6-氰基-7-硝基喹喔啉-2,3-二酮（CNQX，10微摩尔）和GABAA受体阻断剂荷包牡丹碱（20微摩尔）的溶液，从药理学上分离出EPSPNMDA。异丙肾上腺素（15微摩尔）的灌注产生了EPSPNMDA的长期增强。用普萘洛尔（10微摩尔）预处理切片可完全阻止异丙肾上腺素的作用，证实了β-肾上腺素能受体的介导作用。这些结果为脊椎动物中枢神经系统中肾上腺素能对兴奋性氨基酸神经传递的调节提供了直接证据。
