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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Smart D, Shaw C, Johnston C F, Halton D W, Fairweather I, Buchanan K D

Comparative Neuroendocrinology Research Group, Queen's University, Belfast, North Ireland, U.K.

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1992 Jul;102(3):477-81. doi: 10.1016/0742-8413(92)90146-x.

Immunoreactivity (IR) towards neuropeptide Y (NPY) and pancreatic polypeptide (PP) has previously been demonstrated in nematodes by immunocytochemistry (ICC). 2. The PP- and NPY-IR in the nematode parasite Ascaris suum were analysed using HPLC and radioimmunoassays (RIAs) specific for either peptide. 3. Quantitative RIA data on the distribution of these IRs in the parasite disagreed with ICC data suggesting that different tissue treatments used in the two techniques lead to different antigen availability. 4. Significant quantities of PP- and NPY-IR were found in the pseudocoelomic fluid suggesting a role for this medium in the transport of peptides. 5. The parasite possessed chromatographically-distinct PP- and NPY-IRs, with each IR being composed of several distinct molecules.

先前通过免疫细胞化学（ICC）已证实在线虫中存在对神经肽Y（NPY）和胰多肽（PP）的免疫反应性（IR）。2. 使用针对这两种肽的高效液相色谱法（HPLC）和放射免疫测定法（RIA）分析了线虫寄生虫猪蛔虫中的PP-IR和NPY-IR。3. 关于这些IR在寄生虫中分布的定量RIA数据与ICC数据不一致，这表明两种技术中使用的不同组织处理导致了不同的抗原可及性。4. 在假体液中发现了大量的PP-IR和NPY-IR，这表明该介质在肽的运输中起作用。5. 该寄生虫拥有色谱上不同的PP-IR和NPY-IR，每种IR由几个不同的分子组成。

Smart D, Shaw C, Johnston C F, Halton D W, Fairweather I, Buchanan K D

Comparative Neuroendocrinology Research Group, Queen's University, Belfast, North Ireland, U.K.

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1992 Jul;102(3):477-81. doi: 10.1016/0742-8413(92)90146-x.

Immunoreactivity (IR) towards neuropeptide Y (NPY) and pancreatic polypeptide (PP) has previously been demonstrated in nematodes by immunocytochemistry (ICC). 2. The PP- and NPY-IR in the nematode parasite Ascaris suum were analysed using HPLC and radioimmunoassays (RIAs) specific for either peptide. 3. Quantitative RIA data on the distribution of these IRs in the parasite disagreed with ICC data suggesting that different tissue treatments used in the two techniques lead to different antigen availability. 4. Significant quantities of PP- and NPY-IR were found in the pseudocoelomic fluid suggesting a role for this medium in the transport of peptides. 5. The parasite possessed chromatographically-distinct PP- and NPY-IRs, with each IR being composed of several distinct molecules.

先前通过免疫细胞化学（ICC）已证实在线虫中存在对神经肽Y（NPY）和胰多肽（PP）的免疫反应性（IR）。2. 使用针对这两种肽的高效液相色谱法（HPLC）和放射免疫测定法（RIA）分析了线虫寄生虫猪蛔虫中的PP-IR和NPY-IR。3. 关于这些IR在寄生虫中分布的定量RIA数据与ICC数据不一致，这表明两种技术中使用的不同组织处理导致了不同的抗原可及性。4. 在假体液中发现了大量的PP-IR和NPY-IR，这表明该介质在肽的运输中起作用。5. 该寄生虫拥有色谱上不同的PP-IR和NPY-IR，每种IR由几个不同的分子组成。