Barthèlèmy C, Fricot G, Hamamah S, Lebos C, Lansac J, Tharanne M J
Unité de Reproduction, C.H.U. Bretonneau, Tours, France.
Int J Fertil. 1992 Nov-Dec;37(6):362-7.
We studied the possible deleterious effects of Percoll gradient centrifugation processing on spermatozoal integrity in 18 semen samples from patients participating in our IVF program, using optical and electron microscopy. Substantial recovery by sperm count was obtained in the 100% Percoll layer (32%), with greater numbers of motile, alive spermatozoa (P < .01). Whether samples studied were from fertile or infertile men, no significant differences, using nonparametric tests with transmission electron microscopy, appeared in spermatozoal membranes, acrosomes, or nuclei either before or after Percoll treatment. No deleterious effects of Percoll use on spermatozoa were detected during the study.
我们使用光学显微镜和电子显微镜，研究了Percoll梯度离心处理对参与我们体外受精项目的患者的18份精液样本中精子完整性可能产生的有害影响。在100%Percoll层中精子数量有显著恢复（32%），有更多活动的活精子（P < 0.01）。无论所研究的样本来自可育男性还是不育男性，使用透射电子显微镜进行非参数检验时，在Percoll处理前后，精子膜、顶体或细胞核均未出现显著差异。在研究过程中未检测到使用Percoll对精子有有害影响。
