Crompton M R, Bartlett T J, MacGregor A D, Manfioletti G, Buratti E, Giancotti V, Goodwin G H
Chester Beatty Laboratories, Institute of Cancer Research, London, UK.
Nucleic Acids Res. 1992 Nov 11;20(21):5661-7. doi: 10.1093/nar/20.21.5661.
This paper describes the characterisation of a novel chicken homeobox gene, Prh, whose encoded homeodomain sequence differs significantly from those of other factors which have been described. As expected, a portion of the encoded protein, containing the homeodomain, is capable of sequence-specific DNA-binding. Outside the homeodomain, Prh, possesses an N-terminal region extremely rich in proline residues and a C-terminal acidic portion, either of which may function as transcription regulatory domains. Since, among the chicken tissues tested, its transcription is restricted to haematopoietic cells, lung and liver, it may function in tissue-specific patterns of gene regulation. Human and murine Prh homologues have also been identified; so it is likely that such genes are a general feature of vertebrate genomes.
本文描述了一种新的鸡源同源异型框基因Prh的特征，其编码的同源异型结构域序列与已描述的其他因子的序列有显著差异。正如预期的那样，编码蛋白的一部分，包含同源异型结构域，能够进行序列特异性的DNA结合。在同源异型结构域之外，Prh具有一个富含脯氨酸残基的N端区域和一个C端酸性部分，其中任何一个都可能作为转录调节结构域发挥作用。由于在所测试的鸡组织中，其转录仅限于造血细胞、肺和肝脏，它可能在基因调控的组织特异性模式中发挥作用。人类和小鼠的Prh同源物也已被鉴定出来；因此，这类基因很可能是脊椎动物基因组的一个普遍特征。
