DOLBY J M
Immunology. 1958 Oct;1(4):328-37.
The histamine-sensitizing factor of was shown by fractionation of extracts to be different from the antigen protecting mice against an intracerebral challenge. Ten per cent methanol precipitates from a calcium chloride extract of broken cells a water-soluble fraction containing the histamine-sensitizing factor and the antigen protecting mice against an intracerebral challenge. Dialysis of this fraction yields a precipitate soluble at H, containing a much greater amount of protective antigen than histamine sensitizing factor and supernatant which has high histamine sensitizing activity and is of poor immunizing potency. Antisera to the material precipitated on dialysis are fairly specific, protecting against an intracerebral challenge but not against an intranasal challenge, and do not contain anti-histamine-sensitizing factor. The supernatant is much less pure; it stimulates antibodies to the histamine-sensitizing factor and also contains both protective antigens. Sera to other fractions have been obtained, however, which protect against an intranasal challenge but do not contain anti-histamine-sensitizing factor. Although mixtures of antigens and mixtures of antibodies were used, sufficient evidence was obtained to distinguish the histamine-sensitizing factor from the two types of protective antigen. The histamine-sensitizing factor is distinct from the agglutinogen, the haemagglutinin and the toxin.
通过对提取物进行分级分离表明，[提取物名称]的组胺致敏因子不同于保护小鼠免受脑内攻击的抗原。用氯化钙破碎细胞提取物经10%甲醇沉淀，得到一种水溶性级分，其中含有组胺致敏因子和保护小鼠免受脑内攻击的抗原。对该级分进行透析，产生一种在pH值[此处H可能有误，推测为pH]时可溶的沉淀物，其保护性抗原的含量远高于组胺致敏因子，还有具有高组胺致敏活性但免疫效力较差的上清液。针对透析时沉淀物质的抗血清具有相当的特异性，能保护小鼠免受脑内攻击，但不能保护免受鼻内攻击且不含抗组胺致敏因子。上清液纯度低得多；它能刺激产生针对组胺致敏因子的抗体，并且也含有两种保护性抗原。然而，已获得针对其他级分的血清，它们能保护小鼠免受鼻内攻击但不含抗组胺致敏因子。尽管使用了抗原混合物和抗体混合物，但仍获得了足够的证据来区分组胺致敏因子与两种类型的保护性抗原。组胺致敏因子不同于凝集原、血凝素和毒素。
