百日咳毒素S1亚基的抗原异质性

Antigenic heterogeneity in subunit S1 of pertussis toxin.

作者信息

Perera V Y, Wardlaw A C, Freer J H

出版信息

J Gen Microbiol. 1986 Feb;132(2):553-6. doi: 10.1099/00221287-132-2-553.

Abstract

Culture supernates containing pertussis toxin (PT) from four strains of Bordetella pertussis were examined for both immunological reactivity and biological activity. PT from all four strains sensitized mice to histamine and toxin was detectable in supernates of all strains when examined by Western blotting with polyclonal antiserum to PT. In supernates of three of the four strains, PT was detectable by an enzyme-linked immunosorbent assay (ELISA) using mouse monoclonal antibody to subunit S1 of PT as the third antibody layer. However, supernates from one strain, 18323, failed to react in ELISA. Electroblots probed with the monoclonal antibody labelled subunit S1 of PT from all strains except that of strain 18323. PT of strain 18323, whilst retaining histamine-sensitizing activity, differed antigenically from that of other strains.

摘要

对来自四株百日咳博德特氏菌的含有百日咳毒素（PT）的培养上清液进行了免疫反应性和生物活性检测。当用抗PT多克隆抗血清进行蛋白质免疫印迹法检测时，所有四株菌的PT均可使小鼠对组胺敏感，并且在所有菌株的上清液中均可检测到毒素。在四株菌中的三株菌的上清液中，使用抗PT亚基S1的小鼠单克隆抗体作为第三抗体层，通过酶联免疫吸附测定（ELISA）可检测到PT。然而，一株菌（18323）的上清液在ELISA中无反应。用单克隆抗体探测的电印迹显示，除18323菌株外，所有菌株的PT均标记有亚基S1。18323菌株的PT虽然保留了组胺致敏活性，但其抗原性与其他菌株不同。