4型腺病毒的细胞内定位。II. 细胞学和荧光素标记抗体研究。
Intracellular localization of type 4 adenovirus. II. Cytological and fluorescein-labelled antibody studies.
作者信息
BOYER G S, DENNY F W, GINSBERG H S
出版信息
J Exp Med. 1959 Jan 1;109(1):85-96. doi: 10.1084/jem.109.1.85.
Abstract
HeLa cell cultures infected with adenovirus type 4 were studied by light and phase-contrast microscopy and by the fluorescent antibody technique for visualization of intracellular antigen. The findings were correlated with the growth curve of infectious virus, determined from companion cultures. The results indicated that those cells undergoing characteristic structural changes observable by light microscopy were those which contain viral antigen. The distribution of the majority of the antigen within the infected cells corresponded to that of the regularly aligned granules and crystal-like masses seen in the nuclei of cells in stained and in unfixed cultures. The production of infectious virus was closely correlated with the development of the characteristic nuclear changes.
摘要
用光学显微镜、相差显微镜以及荧光抗体技术对感染4型腺病毒的海拉细胞培养物进行研究，以观察细胞内抗原。研究结果与从对照培养物中测定的感染性病毒生长曲线相关。结果表明，那些通过光学显微镜可观察到发生特征性结构变化的细胞就是含有病毒抗原的细胞。感染细胞内大多数抗原的分布与在染色和未固定培养物的细胞核中所见的规则排列颗粒和晶体样物质的分布一致。感染性病毒的产生与特征性核变化的发展密切相关。
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