Anderluh G, Krizaj I, Strukelj B, Gubensek F, Macek P, Pungercar J
Department of Biology, Biotechnical Faculty, University of Ljubljana, Slovenia.
Toxicon. 1999 Oct;37(10):1391-401. doi: 10.1016/s0041-0101(99)00082-3.
The multigene family of equinatoxins, pore-forming proteins from sea anemone Actinia equina, has been studied at the protein and gene levels. We report the cDNA sequence of a new, sphingomyelin inhibited equinatoxin, EqtIV. The N-terminal sequences of natural Eqt I and III were also determined, confirming two isoforms of EqtI, differing at position 13. The number of Eqt genes determined by Southern blot hybridization was found to be more than five, indicating that Eqts belong to a multigene family.
海葵毒素多基因家族是来自海葵Actinia equina的成孔蛋白，已在蛋白质和基因水平上进行了研究。我们报道了一种新的、受鞘磷脂抑制的海葵毒素EqtIV的cDNA序列。还确定了天然Eqt I和III的N端序列，证实了EqtI的两种异构体，在第13位有所不同。通过Southern印迹杂交确定的Eqt基因数量超过五个，表明Eqt属于一个多基因家族。
