Grzegorzewska A E, Moore H L, Chen T W, Nolph K D
Department of Medicine, University of Missouri Health Sciences Center, Harry S. Truman Veterans Administration, Columbia.
Adv Perit Dial. 1992;8:26-9.
In our previous rat studies (Kidney Int. 1991; 39: 608-617) we evaluated peritoneal clearances (Cp) representing near exclusively diffusive CO2 transfer: for isosmotic (0.37% dextrose) and hyperosmotic (15% dextrose) solutions with pH 7.2-7.3, CpCO2 were 1.20 +/- 0.08 and 1.84 +/- 0.04 ml/min, respectively. In the present studies we have compared Cp of CO2 gas and HCO3- in anesthetized rats (n = 22) using solutions with dextrose contents as mentioned above but with pH 6.5 or 7.6; we have also evaluated how much Cp CO2 measurements obtained with solutions at these pH values differ from Cp shown earlier with the solutions of pH 7.2-7.3. Cp of CO2 gas and HCO3- were significantly higher under hyperosmotic than isosmotic conditions. The use of solutions of pH different from 7.2-7.3 resulted in higher Cp of CO2 gas: with isosmotic solutions of pH 6.5 and 7.6 mean increases were 25 and 75%, respectively; with hyperosmotic solutions respective increases were 45 and 134%. We conclude that dialysis solution osmolality and pH significantly change Cp of CO2 gas and HCO3- in the rat. For evaluation of peritoneal blood flow from diffusive transfer parameters of CO2 gas, smaller overestimation can be expected when dialysis solution pH is slightly under-than overadjusted compared to blood pH.
在我们之前的大鼠研究中（《肾脏病国际》1991年；39卷：608 - 617页），我们评估了几乎完全代表扩散性二氧化碳转运的腹膜清除率（Cp）：对于pH值为7.2 - 7.3的等渗（0.37%葡萄糖）和高渗（15%葡萄糖）溶液，二氧化碳的腹膜清除率（CpCO2）分别为1.20±0.08和1.84±0.04毫升/分钟。在本研究中，我们比较了麻醉大鼠（n = 22）中二氧化碳气体和碳酸氢根的腹膜清除率，使用上述葡萄糖含量但pH值为6.5或7.6的溶液；我们还评估了在这些pH值下用溶液获得的二氧化碳腹膜清除率测量值与之前pH值为7.2 - 7.3的溶液所显示的腹膜清除率有多大差异。在高渗条件下，二氧化碳气体和碳酸氢根的腹膜清除率显著高于等渗条件。使用pH值不同于7.2 - 7.3的溶液会导致二氧化碳气体的腹膜清除率更高：对于pH值为6.5和7.6的等渗溶液，平均增加分别为25%和75%；对于高渗溶液，相应的增加分别为45%和134%。我们得出结论，透析液的渗透压和pH值会显著改变大鼠体内二氧化碳气体和碳酸氢根的腹膜清除率。为了从二氧化碳气体的扩散转运参数评估腹膜血流量，与血液pH值相比，当透析液pH值略低于而非过度调整时，预计高估程度会较小。
