Hsu K, Wang D, Shen C Y, Chiang C H
Department of Internal Medicine, Tri-Service General Hospital, Taipei, Taiwan, R.O.C.
J Formos Med Assoc. 1992 Oct;91(10):955-61.
The role of platelets in acute lung injury has not been well defined. In the present study of isolated perfused rat lungs, a significant increase in pulmonary arterial pressure (PAP), lung weight and the lung lavage fluid protein concentration occurred when 3 mL of a CaCl2 suspension (25 mg/mL) was mixed with blood-KHB (Krebs-Henseleit Buffer) perfusate; or when platelets were added instead of blood. However, such an increase was not observed (p > 0.05) when only KHB was used. When a comparatively small amount (0.75 mL) of the CaCl2 suspension (50 mg/mL) was added to the KHB perfusate, PAP, lung weight and the lung lavage protein concentration increased only when PAF (platelet activating factor) and platelets were both added before, but not separately. The above phenomena show that platelets are actively involved in the mechanism of acute lung injury and play an extremely important role in this microembolic model.
血小板在急性肺损伤中的作用尚未得到明确界定。在本项关于离体灌注大鼠肺的研究中，当将3 mL氯化钙悬浮液（25 mg/mL）与血液 - 克氏 - 亨氏缓冲液（Krebs - Henseleit Buffer）灌注液混合时，或者当加入血小板而非血液时，肺动脉压（PAP）、肺重量以及肺灌洗液蛋白浓度均显著增加。然而，当仅使用克氏 - 亨氏缓冲液时，未观察到这种增加（p > 0.05）。当向克氏 - 亨氏缓冲液灌注液中加入相对少量（0.75 mL）的氯化钙悬浮液（50 mg/mL）时，只有在预先同时加入血小板活化因子（PAF）和血小板而非单独加入时，肺动脉压、肺重量和肺灌洗蛋白浓度才会增加。上述现象表明，血小板积极参与急性肺损伤机制，并且在这种微栓塞模型中发挥极其重要的作用。
