Chadburn A, Inghirami G, Knowles D M
Department of Pathology, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Hematol Pathol. 1992;6(4):193-202.
Cell surface antigens, including CD71 (T9), CD38 (T10), HLA-DR, CD25 (IL2-R), CD15 (LeuM1), CD30 (Ki-1), epithelial membrane antigen (EMA), and CD11c (LeuM5), have been identified on the surface of neoplastic T-cells. The significance of this expression is unknown since the expression of these antigens by benign T cells has not been fully investigated. In this study the kinetics, temporal relation and hierarchy of expression of these eight cell surface antigens by purified normal peripheral blood T cells following activation with phytohemagglutinin (PHA) were investigated using one- and two-color flow cytometry. All eight antigens were expressed in a hierarchical manner following activation of normal peripheral blood T cells with PHA. The sequence of antigen expression and the initial time point of this expression was: CD38, < 24 h; CD71, CD25, 24 h; EMA, HLA-DR, CD15, 48-72 h; CD30, 72 h; and CD11c, 96-120 h. The maximum percentage of T cells expressing each antigen and the time point of maximum expression was: CD38 96% at 14 and 17 days; CD71 88%, CD25 94%, EMA 55%, and CD30 31% at 96 h; CD15 56% at 120 h; HLA-DR 30% at 168 h; and CD11c 42% at 240 h. The expression of these 8 antigens clustered into six distinct immunophenotypic constellations: Group I: None; Group II: CD38 with CD71 and/or CD25; Group III: CD38, CD71, CD25 with HLA-DR, CD15 and/or EMA; Group IV: CD38, CD71, CD25, EMA, HLA-DR with CD15, CD30 and/or CD11c; Group V: CD38, CD25, CD11c with CD71, EMA and/or HLA-DR; Group VI: CD38 with CD25 and/or CD11c. Finally, EMA and CD11c were preferentially expressed by CD4 and CD8 T cells, respectively. In summary, these results demonstrate that all eight antigens (1) are associated with T-cell activation, (2) are expressed in a hierarchical manner following activation, and (3) that this expression clusters into distinct immunophenotypic constellations.
肿瘤性T细胞表面已鉴定出多种细胞表面抗原，包括CD71（T9）、CD38（T10）、HLA-DR、CD25（IL2-R）、CD15（LeuM1）、CD30（Ki-1）、上皮膜抗原（EMA）和CD11c（LeuM5）。由于尚未充分研究良性T细胞对这些抗原的表达情况，因此这种表达的意义尚不清楚。在本研究中，使用单克隆抗体和双色流式细胞术研究了用植物血凝素（PHA）激活后纯化的正常外周血T细胞对这八种细胞表面抗原的表达动力学、时间关系和表达层次。用PHA激活正常外周血T细胞后，所有八种抗原均按层次表达。抗原表达顺序及开始表达的时间点为：CD38，<24小时；CD71、CD25，24小时；EMA、HLA-DR、CD15，48 - 72小时；CD30，72小时；CD11c，96 - 120小时。表达每种抗原的T细胞的最大百分比及最大表达时间点为：CD38在14天和17天时为96%；CD71在96小时时为88%，CD25为94%，EMA为55%，CD30为31%；CD15在120小时时为56%；HLA-DR在168小时时为30%；CD11c在240小时时为42%。这8种抗原的表达聚集成6种不同的免疫表型组合：第一组：无；第二组：CD38与CD71和/或CD25；第三组：CD38、CD71、CD25与HLA-DR、CD15和/或EMA；第四组：CD38、CD71、CD25、EMA、HLA-DR与CD15、CD30和/或CD11c；第五组：CD38、CD25、CD11c与CD71、EMA和/或HLA-DR；第六组：CD38与CD25和/或CD11c。最后，EMA和CD11c分别优先由CD4和CD8 T细胞表达。总之，这些结果表明，所有八种抗原（1）与T细胞活化相关，（2）活化后按层次表达，（3）这种表达聚集成不同的免疫表型组合。
