Chen Y S
Department of Otolaryngology, Taiwan Provincial Taipei Hospital.
J Formos Med Assoc. 1992 Sep;91 Suppl 3:S246-50.
When regenerating from an injury, fluid containing nerve growth-promoting components such as the nerve growth factor (NGF) will accumulate around the stump of a cut nerve within 24 hours. It has been found that this fluid also contains fibrin. To evaluate the effect of fibrin on nerve regeneration, we cut both facial nerves of 40 guinea pigs and sutured both ends of the nerve stumps to silicone tubes. The distance between the stumps were kept at around 8 mm in the silicone chamber. Autogenous fibrin isolated from the blood of each guinea pig was injected into the silicone chamber on the left side, while lactate Ringer's solution was injected into the silicone chamber on the right side as a control. The status of nerve regeneration in both sides of the silicone chambers were later evaluated at 3 and 5 weeks. Histological study revealed that after regeneration for 3 weeks, the total axon number and the fascicular number increased significantly in the fibrin-filled chamber. However, after 5 weeks, there was no significant difference in nerve regeneration between the two groups.
在从损伤中再生时，含有神经生长促进成分（如神经生长因子，NGF）的液体在24小时内会在切断神经的残端周围积聚。已发现这种液体中还含有纤维蛋白。为评估纤维蛋白对神经再生的影响，我们切断了40只豚鼠的双侧面神经，并将神经残端的两端缝合到硅胶管上。在硅胶腔内，残端之间的距离保持在约8毫米。从每只豚鼠血液中分离出的自体纤维蛋白被注入左侧的硅胶腔，而乳酸林格氏液被注入右侧的硅胶腔作为对照。随后在3周和5周时评估硅胶腔两侧的神经再生状况。组织学研究显示，再生3周后，充满纤维蛋白的腔内总轴突数和束状结构数量显著增加。然而，5周后，两组之间的神经再生没有显著差异。
