Aronson J, Shen X C, Gao G G, Miller F, Quattlebaum T, Skinner R A, Badger T M, Lumpkin C K
Department of Orthopaedics, University of Arkansas for Medical Sciences, Little Rock, USA.
J Orthop Res. 1997 Jul;15(4):563-9. doi: 10.1002/jor.1100150412.
These studies were conducted to compare the local cellular proliferation patterns in the rat tibia during distraction osteogenesis with those during nondistracted fracture healing. Bone specimens from distraction osteogenesis and nondistracted fracture groups were analyzed 2, 10, and 20 days after surgery. Proliferation was determined by metabolic labeling with [3H]thymidine and by immunocytochemistry with an antibody to proliferating cell nuclear antigen. Videomicroscopy was used to count the cells staining positively within specified regions. The number of cells incorporating [3H]thymidine was positively correlated (r2 = 0.78) with the number of proliferating cell nuclear antigen positive cells on alternating serial slides. At day 2, the latter cells were largely confined to the bone marrow and periosteum in both groups, and the cell numbers per mm2 were also equivalent. At days 10 and 20, the proliferating cell nuclear antigen positive cells predominated in both the proximal and distal primary matrix front zones in the distraction osteogenesis group. In contrast, the proliferating cell nuclear antigen positive cells in the nondistracted fracture group were scattered throughout the healing area. Significantly more of these cells were in the primary matrix front zones than in any location within the nondistracted fracture-healing area. The number of these cells in the bone marrow adjacent to the surgical area declined from day 2 to day 10 in both groups. The results suggest that (a) proliferating cell nuclear antigen immunostaining is a reliable indicator of cycling cells; (b) by day 10, distraction osteogenesis is characterized by a zone-specific pattern of proliferating cells; and (c) distraction osteogenesis prolongs the stimulation of proliferation within the gap after fracture.
进行这些研究是为了比较大鼠胫骨在牵张成骨过程中的局部细胞增殖模式与非牵张性骨折愈合过程中的局部细胞增殖模式。在术后2天、10天和20天对牵张成骨组和非牵张性骨折组的骨标本进行分析。通过用[3H]胸腺嘧啶核苷进行代谢标记以及用抗增殖细胞核抗原抗体进行免疫细胞化学来确定增殖情况。使用视频显微镜对指定区域内染色阳性的细胞进行计数。在交替的连续切片上，掺入[3H]胸腺嘧啶核苷的细胞数量与增殖细胞核抗原阳性细胞数量呈正相关（r2 = 0.78）。在第2天，两组中的后一种细胞主要局限于骨髓和骨膜，每平方毫米的细胞数量也相当。在第10天和20天，牵张成骨组近端和远端初级基质前沿区域中增殖细胞核抗原阳性细胞占主导。相比之下，非牵张性骨折组中增殖细胞核抗原阳性细胞分散在整个愈合区域。这些细胞在初级基质前沿区域中的数量明显多于非牵张性骨折愈合区域内的任何位置。两组中手术区域附近骨髓中的这些细胞数量从第2天到第10天均下降。结果表明：（a）增殖细胞核抗原免疫染色是循环细胞的可靠指标；（b）到第10天，牵张成骨的特征是增殖细胞具有区域特异性模式；（c）牵张成骨延长了骨折后间隙内增殖的刺激时间。
