人类D2多巴胺受体cDNA及基因的克隆
Cloning of the cDNA and gene for a human D2 dopamine receptor.
作者信息
Grandy D K, Marchionni M A, Makam H, Stofko R E, Alfano M, Frothingham L, Fischer J B, Burke-Howie K J, Bunzow J R, Server A C
机构信息
Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland 97201.
出版信息
Proc Natl Acad Sci U S A. 1989 Dec;86(24):9762-6. doi: 10.1073/pnas.86.24.9762.
A clone encoding a human D2 dopamine receptor was isolated from a pituitary cDNA library and sequenced. The deduced protein sequence is 96% identical with that of the cloned rat receptor with one major difference: the human receptor contains an additional 29 amino acids in its putative third cytoplasmic loop. Southern blotting demonstrated the presence of only one human D2 receptor gene. Two overlapping phage containing the gene were isolated and characterized. DNA sequence analysis of these clones showed that the coding sequence is interrupted by six introns and that the additional amino acids present in the human pituitary receptor are encoded by a single exon of 87 base pairs. The involvement of this sequence in alternative splicing and its biological significance are discussed.
从垂体cDNA文库中分离出一个编码人类D2多巴胺受体的克隆并进行测序。推导的蛋白质序列与克隆的大鼠受体的序列有96%的同一性，主要区别在于：人类受体在其假定的第三个细胞质环中含有另外29个氨基酸。Southern印迹法显示仅存在一个人类D2受体基因。分离并鉴定了两个含有该基因的重叠噬菌体。这些克隆的DNA序列分析表明，编码序列被六个内含子打断，并且人类垂体受体中存在的额外氨基酸由一个87个碱基对的单一外显子编码。讨论了该序列在可变剪接中的作用及其生物学意义。
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