Okada S, Hiroshige R, Tanaka M, Murai M, Kimura T
Eisei Shikenjo Hokoku. 1990(108):147-9.
The raw material of hydrocortisone acetate was examined for preparation of the "Hydrocortisone Acetate Reference Standard". Analytical results were as follows: E1%1cm (242 nm) 403; IR spectrum indicates specific absorption at 3424, 1745, 1721, 1630, and 1374 cm-1; optical rotation +164.4 degrees (in dioxane); melting point 219.5 degrees (decomposition); loss on drying 0.16%; TLC and HPLC analyses indicated one and three impurities, respectively; assay by HPLC was 99.7% against the previous Reference Standard. Based on the results, the present raw material was authorized to be the Reference Standard of the National Institute of Hygienic Sciences.
对醋酸氢化可的松原料进行了检测，以制备“醋酸氢化可的松对照品”。分析结果如下：E1%1cm（242nm）为403；红外光谱表明在3424、1745、1721、1630和1374cm-1处有特定吸收；旋光度为+164.4度（在二氧六环中）；熔点为219.5度（分解）；干燥失重为0.16%；薄层色谱法和高效液相色谱法分析分别表明有1种和3种杂质；相对于先前的对照品，高效液相色谱法测定含量为99.7%。根据这些结果，该原料被批准为国立卫生科学研究所的对照品。
