Murai M, Kakehi H, Komatsu H, Ishimitsu S, Okada S
Eisei Shikenjo Hokoku. 1991(109):173-5.
The raw material of riboflavin was examined for the preparation of the "Riboflavin Reference Standard (Control 891)". Analytical data obtained were as follows: melting point, 238.2 degrees C (decomposition); absorbance, E1%1cm = 859 (267 nm), 277 (372 nm), 327 (445 nm); infrared spectrum, same as Riboflavin Reference Standard (Control 872); thin-layer chromatography, 5 impurities were detected; high-performance liquid chromatography, 11 impurities were detected; loss on drying, 0.25%; optical rotation, [alpha]20D = -133.6 degrees; solubility, less than 16 min; assay, 100.1% against Riboflavin Reference Standard (Control 872). Based on the above results, this raw material was authorized to be the Reference Standard of the National Institute of Hygienic Sciences.
对用于制备“核黄素标准品（对照品891）”的核黄素原料进行了检验。获得的分析数据如下：熔点238.2℃（分解）；吸光度，E1%1cm = 859（267nm），277（372nm），327（445nm）；红外光谱，与核黄素标准品（对照品872）相同；薄层色谱法，检测到5种杂质；高效液相色谱法，检测到11种杂质；干燥失重0.25%；旋光度，[α]20D = -133.6°；溶解度，小于16分钟；含量测定，相对于核黄素标准品（对照品872）为100.1%。基于上述结果，该原料被批准作为国立卫生科学研究所的标准品。
