Murai M, Kakehi H, Komatsu H, Ishimitsu S, Okada S
Eisei Shikenjo Hokoku. 1991(109):154-6.
The raw material of methoxalene was examined for the preparation of the "Methoxalene Reference Standard". Analytical data obtained were as follows: ultraviolet spectrum, lambda max = 300, 249 and 218 nm; absorbance, E1cm(1%) (300 nm) = 559; infrared spectrum, same as that of USP Reference Standard of Methoxalene; melting point, 148 degrees C; thin-layer chromatography, no impurities were detected until 50 micrograms; high-performance liquid chromatography (HPLC), no impurities were detected, water, 0.04%; assay, 99.9% by absorbance method and 100.4% by the HPLC method in terms of the USP Reference Standard. Based on the above results, this raw material was authorized to be the Reference Standard of the National Institute of Hygienic Sciences.
对甲氧沙林的原料进行了检验，以制备“甲氧沙林对照品”。获得的分析数据如下：紫外光谱，最大吸收波长λmax = 300、249和218nm；吸光度，E1cm(1%) (300nm) = 559；红外光谱，与美国药典甲氧沙林对照品相同；熔点，148℃；薄层色谱法，直至50微克均未检测到杂质；高效液相色谱法（HPLC），未检测到杂质，水分，0.04%；含量测定，以美国药典对照品计，吸光法为99.9%，HPLC法为100.4%。基于上述结果，该原料被批准为国立卫生科学研究所的对照品。
