5-溴脱氧尿苷可能会改变胚胎胰腺的分化程序。
5-bromodeoxyuridine may alter the differentiative program of the embryonic pancreas.
作者信息
Githens S, Pictet R, Phelps P, Rutter W J
出版信息
J Cell Biol. 1976 Nov;71(2):341-56. doi: 10.1083/jcb.71.2.341.
Abstract
The thymidine analog, 5-bromodeoxyuridine (BrdU), inhibits the differentiation of the acinar cells of the embryonic rat pancreas, while having little effect on the growth of the tissue. The BrdU-treated pancreas contains elevated alkaline phosphatase and carbonic anhydrase activities, and, unlike the normal pancreas, contains numerous extracellular fluid-filled vacuoles, surrounded by ductlike cells. Both alkaline phosphatase and carbonic anhydrase activities are located preferentially in the ductlike cells lining the vacuoles. The biochemical, morphological, and functional features of these epithelial cells are therefore characteristic of the normal pancreatic duct cell. Thus, in the exocrine pancreas, BrdU seems to alter the normal program of differentiation by favoring the functional duct cells while inhibiting the differentiation of acinar cells.
摘要
胸腺嘧啶核苷类似物5-溴脱氧尿苷（BrdU）可抑制胚胎大鼠胰腺腺泡细胞的分化，而对该组织的生长影响甚微。经BrdU处理的胰腺碱性磷酸酶和碳酸酐酶活性升高，且与正常胰腺不同，含有许多被导管样细胞包围的充满细胞外液的空泡。碱性磷酸酶和碳酸酐酶活性均优先位于空泡内衬的导管样细胞中。因此，这些上皮细胞的生化、形态和功能特征是正常胰腺导管细胞的特征。所以，在外分泌胰腺中，BrdU似乎通过促进功能性导管细胞而抑制腺泡细胞的分化来改变正常的分化程序。
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