Buanes T, Grotmol T, Landsverk T, Ridderstråle Y, Raeder M G
Acta Physiol Scand. 1986 Nov;128(3):437-44. doi: 10.1111/j.1748-1716.1986.tb07997.x.
Carbonic anhydrase is essential for the pancreatic secretion of NaHCO3. To localize the distribution of carbonic anhydrase in the exocrine gland and, hence, identify the potential cellular source of secreted NaHCO3, histochemical staining of carbonic anhydrase was carried out on pancreatic biopsies obtained from six pigs at both supramaximal intravenous secretin stimulation and at secretory rest. Tissue staining, using Hansson's technique, revealed a strong staining both in membranes and cytoplasm in the duct cells and a weaker cytoplasmic staining in the acinar cells. Staining reaction was abolished by 10(-5) mol l-1 acetazolamide. Duct cells, accordingly, seem to be responsible for NaHCO3 secretion, while acinar cells appear unlikely to contribute substantially to NaHCO3 secretion.
碳酸酐酶对于胰腺分泌碳酸氢钠至关重要。为了定位碳酸酐酶在外分泌腺中的分布，从而确定分泌的碳酸氢钠的潜在细胞来源，我们对6头猪在静脉注射超最大剂量促胰液素刺激时和分泌静止时获取的胰腺活检组织进行了碳酸酐酶的组织化学染色。使用汉森技术进行的组织染色显示，导管细胞的细胞膜和细胞质均有强染色，腺泡细胞的细胞质染色较弱。10⁻⁵摩尔/升乙酰唑胺可消除染色反应。因此，导管细胞似乎负责碳酸氢钠的分泌，而腺泡细胞似乎不太可能对碳酸氢钠分泌有实质性贡献。
