Ferguson R, Anderson S M, Schmidtke J R, Simmons R L
J Immunol. 1976 Dec;117(6):2150-7.
Vibrio cholerae neuraminidase (VCN))12.5 units/2 X 10(6) cells/ml) continuously present for a standard 5-day MLC will significant (p less than 0.02) increase the cytotoxic activity generated by a given number of responding spleen cells without reducing the specificity. Heat-inactiviated VCN produced no such augmentation. This augmented cytotoxicity could be reproduced by preincubating (1 hr) the responding spleen cells with VCN (25 units/2.5 X 10(6) cells/ml) before addition of stimulating spleen cells. Preincubating the stimulator spleen cells with VCN had no effect. VCN preincubation of target cells or presensitized effector cells produced no augmentation. The addition of soluble VCN to the killing assay also did not increase cytotoxicity. Thus, VCN acts only during the generation of specifically sensitized cytotoxic T cells. When the effect of VCN on MLC reactivity, cell recovery and total cytotoxicity (lytic units/10(6) cells) were compared, it became apparent that VCN increases the proliferation of responder cells after stimulation resulting in both an increased number of cells and also an increase in the proportion of specifically sensitized cytotoxic cells in the culture. VCN treatment of responder cell membrane apparently permits a more ready response to allogenic antigens in culture facilitating both increased proliferation and the increased development of specific cytotoxic killers.
霍乱弧菌神经氨酸酶（VCN，12.5单位/2×10⁶细胞/毫升）在标准的5天混合淋巴细胞培养（MLC）中持续存在，会显著（p＜0.02）提高给定数量的反应性脾细胞产生的细胞毒性活性，且不降低特异性。热灭活的VCN不会产生这种增强作用。在加入刺激脾细胞之前，用VCN（25单位/2.5×10⁶细胞/毫升）对反应性脾细胞进行预孵育（1小时），可重现这种增强的细胞毒性。用VCN对刺激脾细胞进行预孵育则没有效果。对靶细胞或预先致敏的效应细胞进行VCN预孵育不会产生增强作用。在杀伤试验中加入可溶性VCN也不会增加细胞毒性。因此，VCN仅在特异性致敏细胞毒性T细胞的产生过程中起作用。当比较VCN对MLC反应性、细胞回收率和总细胞毒性（裂解单位/10⁶细胞）的影响时，很明显VCN会增加刺激后反应细胞的增殖，导致细胞数量增加，同时培养物中特异性致敏细胞毒性细胞的比例也增加。VCN处理反应细胞膜显然能使细胞对培养中的同种异体抗原更容易产生反应，促进增殖增加和特异性细胞毒性杀伤细胞的发育增加。
