A non-radiolabelled in situ hybridization method for the detection of epidermal cytokine mRNA.
作者信息
Howie S E, Aldridge R D, McVittie E, Thornton E, Ramage E, Hunter J A
机构信息
Department of Dermatology, University of Edinburgh, Scotland.
出版信息
Exp Dermatol. 1992 Dec;1(5):230-5. doi: 10.1111/j.1600-0625.1992.tb00081.x.
Abstract
We describe a non-radiolabelled method for the in situ detection of epidermal cytokine mRNA in paraffin sections of skin biopsies from sites of nickel contact sensitivity. The method uses fluorescein isothiocyanate-labelled oligonucleotide exon probe cocktails.
摘要
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