巨球蛋白血症。III. 巯基乙醇对巨球蛋白抗原结构的影响。
Macroglobulinemia. III. The effect of mercaptoethanol on the antigenic structure of macroglobulins.
作者信息
KORNGOLD L, VAN LEEUWEN G
出版信息
J Exp Med. 1959 Jul 1;110(1):1-8. doi: 10.1084/jem.110.1.1.
The monomers obtained by treating gamma(1)-macroglobulins with mercaptoethanol have proved to be antigenically different from normal 7S 7gamma-globulin. The depolymerization of the macroglobulins resulted in the loss of several antigenic determinants, although the monomers still cross-reacted with antisera against macroglobulins. Reaggregation of the monomers occasionally resulted in the reconstitution of some or all of the antigenic determinants that were lost during depolymerization. Repeated freezing and thawing of pathological macroglobulins in iodoacetate resulted in their complete antigenic destruction. Repeated freezing and thawing of one of the monomers in excess iodoacetate resulted in the degradation to a protein antigenically indistinguishable from Bence Jones protein. The other two monomers studied were stable under these conditions.
用巯基乙醇处理γ(1)-巨球蛋白所得到的单体已被证明在抗原性上与正常的7S 7γ-球蛋白不同。巨球蛋白的解聚导致了几种抗原决定簇的丧失，尽管单体仍与抗巨球蛋白抗血清发生交叉反应。单体的重新聚集偶尔会导致在解聚过程中丧失的部分或全部抗原决定簇的重建。在碘乙酸盐中对病理性巨球蛋白进行反复冻融导致其完全抗原性破坏。在过量碘乙酸盐中对其中一种单体进行反复冻融导致其降解为一种在抗原性上与本斯·琼斯蛋白无法区分的蛋白质。所研究的另外两种单体在这些条件下是稳定的。
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