Ward M, Wilson L J, Kodama K H, Rey M W, Berka R M
Genencor Inc., South San Francisco, CA 94080.
Biotechnology (N Y). 1990 May;8(5):435-40. doi: 10.1038/nbt0590-435.
We have extended the work on chymosin production in Aspergillus by constructing an expression vector in which the cDNA encoding bovine prochymosin B was fused in frame immediately following the codon for the last amino acid of the A. awamori glucoamylase (glaA) gene. Transformation of A. awamori with this plasmid led to the secretion of considerably higher amounts of chymosin than obtained with previous chymosin expression vectors. We present evidence that mature chymosin is autocatalytically released from the glucoamylase-chymosin fusion protein after secretion.
我们通过构建一个表达载体扩展了在曲霉中生产凝乳酶的工作，该表达载体中编码牛凝乳酶原B的cDNA紧接泡盛曲霉糖化酶（glaA）基因最后一个氨基酸的密码子之后进行读框融合。用该质粒转化泡盛曲霉导致分泌的凝乳酶量比使用先前的凝乳酶表达载体时获得的量高得多。我们提供的证据表明，成熟的凝乳酶在分泌后从糖化酶-凝乳酶融合蛋白中自动催化释放。
