Contreras R, Carrez D, Kinghorn J R, van den Hondel C A, Fiers W
Laboratory of Molecular Biology, State University, Gent, Belgium.
Biotechnology (N Y). 1991 Apr;9(4):378-81. doi: 10.1038/nbt0491-378.
We have designed an expression vector for the secretion of human interleukin-6 (hIL-6) in which the mature protein is fused through a spacer peptide, containing a KEX-2 like protein processing signal, to the entire Aspergillus niger glucoamylase (glaA) gene. Transformation of Aspergillus nidulans with this vector results in fungal strains secreting equimolar amounts of the glucoamylase and IL-6 proteins. The KEX2-type processing signal, Lys-Arg, is recognized and cleaved efficiently by an enzyme present in A. nidulans resulting in the secretion of an authentic mature hIL-6 protein at levels of up to 5 mg/l.
我们设计了一种用于分泌人白细胞介素-6（hIL-6）的表达载体，其中成熟蛋白通过一个含有类KEX-2蛋白加工信号的间隔肽与黑曲霉糖化酶（glaA）全基因融合。用该载体转化构巢曲霉，产生的真菌菌株可分泌等摩尔量的糖化酶和IL-6蛋白。构巢曲霉中存在的一种酶可有效识别并切割KEX2型加工信号Lys-Arg，从而以高达5mg/l的水平分泌出真实的成熟hIL-6蛋白。
