Lleonart R, Näf D, Browning H, Weissmann C
Institut für Molekularbiologie I, Universität Zürich, Switzerland.
Biotechnology (N Y). 1990 Dec;8(12):1263-7. doi: 10.1038/nbt1290-1263.
We describe a specific and quantitative novel assay for biologically active human type I interferon (IFN), the MxR assay. It is based on a Vero cell line containing multiple copies of a hybrid gene consisting of the murine Mx promoter, which is responsive to type I IFN, linked to the human growth hormone (hGH) transcription unit. Exposure of this cell line to IFN-alpha or -beta for 12-48 hours results in the production of hGH that is measured by a commercially available radio-immune assay. The response to IFN-alpha is dose-dependent between 3 and 1000 units/ml. There is no response to TNF, IL-1 and a number of other cytokines and growth factors, and only a negligible response to IFN-gamma.
我们描述了一种针对生物活性人I型干扰素（IFN）的特异性定量新检测方法——MxR检测法。它基于一种Vero细胞系，该细胞系含有一个杂交基因的多个拷贝，该杂交基因由对I型干扰素敏感的小鼠Mx启动子与人生长激素（hGH）转录单位相连组成。将此细胞系暴露于IFN-α或 -β 12至48小时会导致产生hGH，可通过市售放射免疫测定法进行测量。对IFN-α的反应在3至1000单位/毫升之间呈剂量依赖性。对TNF、IL-1和许多其他细胞因子及生长因子无反应，对IFN-γ只有微不足道的反应。
