Mohr G, Esser K
Lehrstuhl für Allgemeine Botanik, Ruhr Universität Bochum, Federal Republic of Germany.
Appl Microbiol Biotechnol. 1990 Oct;34(1):63-70. doi: 10.1007/BF00170925.
Wild-type strains of Aspergillus niger were transformed with integrative vectors. The number of stable transformants varied from approximately 20-30/micrograms up to 17,000/micrograms using acetamide and hygromycin B selection, respectively. The introduction of deletions of 5' and 3' non-coding regions of the acetamidase gene (amdS+) revealed that these sequences influenced the number of transformants. The molecular characterization of A. niger transformants revealed that several copies of the vectors were tandemly integrated into the nuclear DNA. These oligomers were stably inherited, even after 100 days of growth on non-selective medium. The expression of the vector-encoded genes was confirmed by evidence from the mRNAs and corresponding proteins encoded by the selectable marker genes.
用整合载体转化黑曲霉的野生型菌株。分别使用乙酰胺和潮霉素B进行筛选时，稳定转化子的数量从约每微克20 - 30个变化到每微克17000个。对乙酰胺酶基因（amdS+）5'和3'非编码区缺失的引入表明，这些序列影响了转化子的数量。黑曲霉转化子的分子特征表明，载体的几个拷贝串联整合到核DNA中。即使在非选择性培养基上生长100天后，这些寡聚物仍能稳定遗传。通过来自选择标记基因编码的mRNA和相应蛋白质的证据证实了载体编码基因的表达。
