Licari P, Winberry L, Swartz R
Biotechnology Engineering Center, Tufts University, Medford, MA 02155.
J Biotechnol. 1991 Feb;17(2):189-93. doi: 10.1016/0168-1656(91)90009-k.
The production of pertussis toxin by Bordetella pertussis was increased by controlling the pH at 7.0 through the addition of sulfuric acid. The more commonly used hydrochloric acid and Tris buffer were observed to be detrimental to toxin yields.
通过添加硫酸将pH值控制在7.0,可增加百日咳博德特氏菌产生百日咳毒素的量。观察发现,更常用的盐酸和Tris缓冲液对毒素产量有不利影响。