通过调整生长培养基的离子组成提高百日咳博德特氏菌的百日咳毒素产量。

Improved pertussis toxin production by Bordetella pertussis through adjusting the growth medium's ionic composition.

作者信息

Frohlich B T, De Bernardez Clark E R, Siber G R, Swartz R W

机构信息

Department of Chemical Engineering, Tufts University, Medford, MA 02155, USA.

出版信息

J Biotechnol. 1995 May 1;39(3):205-19. doi: 10.1016/0168-1656(95)00013-g.

DOI:10.1016/0168-1656(95)00013-g

PMID:7766402

Abstract

Ionic composition and total ionic concentration of the growth medium were important factors in limiting productivities in aerated reactors used for the production of pertussis toxin and other antigens by Bordetella pertussis. Salt concentration has opposing effects on cell growth of wild-type B. pertussis and specific toxin formation. Sodium ion concentrations below 140 mM correlated with a precipitous decline in specific yields of pertussis toxin, an otherwise growth-associated product. High salt concentrations in the medium resulted in lower final cell concentrations but did not affect initial growth rates. A new medium is proposed that allows a 60 to 70% increase in both cell and toxin yields by replacing the sodium chloride in the 'cyclodextrin liquid' (CL) medium with additional monosodium glutamate which provides both the sodium and the carbon and energy source.

摘要

用于百日咳博德特氏菌生产百日咳毒素和其他抗原的曝气反应器中，生长培养基的离子组成和总离子浓度是限制生产力的重要因素。盐浓度对野生型百日咳博德特氏菌的细胞生长和特定毒素形成具有相反的影响。钠离子浓度低于140 mM与百日咳毒素特定产量的急剧下降相关，百日咳毒素是一种原本与生长相关的产物。培养基中高盐浓度导致最终细胞浓度较低，但不影响初始生长速率。提出了一种新的培养基，通过用额外的谷氨酸钠替代“环糊精液体”（CL）培养基中的氯化钠，使细胞和毒素产量提高60%至70%，谷氨酸钠提供了钠以及碳和能量来源。